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12 Oct 2021 04:17:06 UTC
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Follistatin / DIY gene therapy project update
Here is the update for the Follistatin gene therapy project.
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video/mp4
Language
English
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Controlling
VIDEO
EASY
easy-diy-fluorescence-microscope
This is a super quick way to make a cheap inverted microscope function as a fluorescent microscope.
If you want to support my projects and get early access to everything you can do that through my patreon.
https://www.patreon.com/DavidIshee
...
https://www.youtube.com/watch?v=4zSMU9wLOpU
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Created
1 year ago
Content Type
Language
video/mp4
English
Controlling
VIDEO
SEPTE
september-project-update
This is the update for September
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https://www.youtube.com/watch?v=ivzu76LbT4M
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Created
1 year ago
Content Type
Language
video/mp4
English
Controlling
VIDEO
APRIL
april-diy-gene-therapy-update
In this video I look through the sequencing results for the new version of my DIY gene therapy and I troubleshoot some issues.
If you want to support my project and get early access to everything you can do that through my patreon.
https://www.patreon.com/DavidIshee
...
https://www.youtube.com/watch?v=pQp6brZVnyQ
Transaction
Created
1 year ago
Content Type
Language
video/mp4
English
Controlling
VIDEO
PURIF
purifying-dna-on-a-giant-scale
This is a my first Gigaprep of the follistatin344 gene therapy DNA (my DiHAC-FST5 construct).
A gigapreps is a protocol that allows you to purify huge amounts of DNA from bacteria all at once. These bacteria have been genetically engineered to make huge amounts of the human follistatin gene, and that's the DNA I'm purifying.
These cell pellets are from my last bioreactor run, they are 3/4 of the total cells produced in a 10L bioreactor run. I produced 4 pellets and already used 1 of them.
This is part of my human DIY gene therapy project, you can see more about it here. https://youtu.be/fpo0YcvgsDE
To get info on all this early check out my patreon.
https://www.patreon.com/DavidIshee
...
https://www.youtube.com/watch?v=A4FYBasHzjM
Transaction
Created
1 year ago
Content Type
Language
video/mp4
English
Controlling
VIDEO
CAS9
cas9-cutting-dna
Cas9 viewed by atomic force microscopy.
Not my work, just super cool
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https://www.youtube.com/watch?v=marMhMz-RAM
Transaction
Created
1 year ago
Content Type
Language
video/mp4
English
Controlling
VIDEO
BIORE
bioreactor-build-for-personal-gene
This is the walk through of building my bioreactor along with what I learned from the first run.
This will allow me to make DNA in quantities needed for adult human genetic modification. This is part of my follistatin project. For the most up to date videos on this project become a patreon patron and get the updates before everyone else. https://www.patreon.com/DavidIshee
Here is a parts list through amazon.
https://www.amazon.com/ideas/amzn1.account.AGB4PNF4HCFRUPOGKC5QMZPNUV7Q/1LA90FCLHRZ0V?ref=idea_share
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https://www.youtube.com/watch?v=-Os5_QSHmOE
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Created
1 year ago
Content Type
Language
video/mp4
English
Controlling
VIDEO
AN IN
Dariiapt4
This is my interview with Dariia Dantseva. She's a Biohacker from Ukraine and she's a part of the biohacker group "The CDC". Here we talk about her work, being censored and deplatformed for teaching science openly and the new show we're both in.
Dariia has a patreon to support her lab and her work you can find it here
https://www.patreon.com/yanelab
And you can support my work at my patreon here
https://www.patreon.com/DavidIshee
Transaction
Created
1 year ago
Content Type
Language
video/mp4
English
Controlling
VIDEO
UNNAT
unnatural-selection-trailer-the-netflix
This is the trailer for season 1 of the upcoming Netflix doc/series, featuring me and a bunch of other biohackers along with some of the best scientists at the cutting edge of biotech. We filmed from 2016-2018 which were crazy years of building this stuff and learning a lot. The trailer came out today but the series itself is out Oct 18th.
This is a big moment for humanity and we have a lot of questions to ask and answer together, I'm super honored to be a part of this conversation and these events. It blows my mind that I got this lucky and met all these amazing people. Not just the biohackers and scientists but also the filmmakers, editors and story tellers, it's been a beautiful few years.
PS. I can see how much weight I've lost in this video. I had a science belly for sure .
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https://www.youtube.com/watch?v=GpWwOnsRDkY
Transaction
Created
1 year ago
Content Type
Language
video/mp4
English
Controlling
VIDEO
BEGIN
beginnings-and-updates
This is mostly an update on the biohacking projects for this month, but also a thank you and several new beginnings.
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https://www.youtube.com/watch?v=5ma0mGREzKo
Transaction
Created
1 year ago
Content Type
Language
video/mp4
English