**The Occurrence, Cultivation, and Chemistry of Psilocybe ovoideocystidiata, a new Bluing Species (Agaricales) from Ohio, Pennsylvania and West Virginia** By Allen, John W., Gartz, Joc Cultivation and analysis of Psilocybe ovoideocystidiata, a new bluing species from Ohio and Bethany West Virginia is presented. Cultivation of this species was demonstrated on hardwood substrate. Analysis of both caps and stems revealed the presence of psilocybin, in most cases psilocin and always low concentrations of baeocystin. Psilocybin, psilocin and baeocystin levels varied in the bluing caps and stems of this new species. The highest concentrations of these alkaloids were found in both naturally grown and cultivated fruiting bodies of Psilocybe ovideocystidiata which, at the present moment is an indigenous species found in Ohio, Pennsylvania and West Virginia.. The relative alkaloidal content of psilocybin, psilocin and baeocystin found in Psilocybe ovoideocystidiata from Ohio was similar to that measured in Psilocybe caerulipes by Leung et al. Recent comparative chemical analysis of both species was unable to be performed due to a denial of specimens through the University of Michigan’s herbarium.

[Psilocybe allenii](https://en.wikipedia.org/wiki/Psilocybe_allenii) – a new bluing species from the Pacific Coast, USA CZECH MYCOLOGY 64(2): 181–195, Dec 7, 2012 ISSN 1805-1421 Authors: [JAN BOROVIČKA](https://www.researchgate.net/profile/Jan_Borovicka) ,[ALAN ROCKEFELLER](https://www.facebook.com/alan.rockefeller) ,PETER G. WERNER In last few years, J.B. received several collections of an unusual Psilocybe spe- cies collected by [John W. Allen](https://www.researchgate.net/profile/John_Allen) in Seattle (WA, USA). Judging from photographs, they were rather similar to P. cyanescens but the pileus margins were not wavy. However, their microscopic characteristics fell within the range of variability of P. cyanescens. As has been recently demonstrated in the group of the European P. serbica M.M. Moser et E. Horak, the macro– and microcharacters can be highly variable in this species (Borovička 2008, Borovička et al. 2011); therefore, one would not place emphasis on the macroscopic differences observed in the collec- tions from Seattle and would attribute them to intraspecific variability of P. cyanescens. However, DNA sequencing of the collections from Seattle and also from the San Francisco Bay Area (Northern California) has revealed that there is a stable 5 base-pair difference in the DNA sequence of the ITS rDNA region (containing ITS1, 5.8S, ITS2 sequences and flanking SSU and LSU regions) which is commonly used for separation of agaric species (Miller & Buyck 2002, Antonín et al. 2009a), including Strophariaceae (Antonín et al. 2009b, Borovička et al. 2011). We there- fore consider the collections to represent a new species.