The film is narrated periodically by Walt Disney. At the age of thirteen, he develops a love for animation and film. In 1919, after returning from his time with the Red Cross, Walt moves in with his older brother Roy and his girlfriend Edna, in Kansas City. Roy later moves to California, staying in a veterans hospital to deal with tuberculosis. Walt is hired as an artist at Pesmen-Rubin Commercial Art Studio, meeting Ub Iwerks. However, Walt and Iwerks are made redundant weeks after when the company's revenue declines. Walt and Iwerks found their own business, Iwerks-Disney Commercial Artists, located in an old barn. They are joined by animators Fred and Hugh Harman, and come up with the "Laugh-O-Grams" to show in Frank L. Newman's theatres. With help from new investors, the Laugh-O-Gram Studio is founded. Walt hires new staff including Friz Freleng and Rudy Ising. The studio struggles financially, and Walt is unable to pay his animators, or keep up his rent. Walt finds solace in a mouse occupying the studio, and contacts New York City-based distributor Margaret Winkler, pitching her a live action-animated Alice series, which Winkler approves of. However, Laugh-O-Gram Studios goes bankrupt shortly after. Walt moves to Los Angeles in 1923, living with his relatives, relying on Roy for financial support. Walt finishes work on the original Alice film, which is approved by Winkler and her husband Charles Mintz. Walt and Roy found the Disney Brothers Studio in 1923, hiring Iwerks and the other animators. Lillian Bounds is hired as an ink artist, Walt eventually falling love with her, and they marry in 1925. Roy also marries Edna. The Alice Comedies are produced, starring Virginia Davis, distributed by Mintz. Mintz, unconfident in Walt's abilities, sends his brother-in-law, George Winkler, to supervise the studio. It also becomes apparent that Mintz continues to own the rights to all of the studio's creations. The Alice Comedies come to an end, and Walt and Iwerks create Oswald the Lucky Rabbit for Universal Studios. Guessing that the Disney brothers are running out of money, George begins talking with the animators, inviting them to join Mintz' new studio for better payment. Iwerks is the only one who refuses the offer. Walt and Lillian travel to New York, hoping to negotiate for a larger fee to make the Oswald shorts. Walt discovers a new contract would surrender all ownership of the Disney studios to Mintz, whose distributor Universal already owns the rights to Oswald, and learns his animators have left. Refusing to agree to the terms, Walt leaves Mintz and Oswald behind. On the train back to Los Angeles, Walt is inspired to create a new character which he owns the rights to. He and Iwerks subsequently create Mickey Mouse. In 1928, Mickey's first short Plane Crazy is screened, and met with a round of applause. Walt, Roy, and Iwerks exit the theatre in celebration, Walt spotting the mouse from the Laugh-O-Gram Studio, reminding him of how far he has come. Insertions during the end credits detail the success Walt, Roy, Iwerks, and the other animators would go on to have in their own careers.

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