Gene Cloning Animation | Genetic Engineering | Basic Science Series Keywords: Gene cloning, molecular cloning, DNA cloning, recombinant DNA, gene manipulation, biotechnology, genetic engineering, PCR amplification, gene isolation, plasmid vectors, DNA ligase, transformation process, selective markers, antibiotic resistance genes, PCR screening, DNA sequencing, biomedical research, disease mechanisms, therapeutic targets, genetic modification, crop improvement, disease-resistant plants, pharmaceutical production, forensic DNA analysis, environmental monitoring. Gene cloning is a revolutionary technique in molecular biology that allows scientists to replicate and manipulate specific genes for various purposes. This comprehensive guide delves into the intricacies of gene cloning, from its fundamental principles to its applications in research, medicine, and biotechnology. Gene cloning, also known as molecular cloning, is the process of isolating and making copies of a specific gene or DNA sequence. This technique involves inserting the gene of interest into a vector, such as a plasmid or a viral genome, and then introducing the vector into a host organism, typically a bacterium or yeast. Once inside the host, the vector replicates along with the host's DNA, producing multiple copies of the inserted gene. How Does Gene Cloning Work? Gene cloning involves several key steps: 1. Isolation of the gene of interest: The target gene is identified and isolated from the source organism's DNA using techniques such as polymerase chain reaction (PCR) or restriction enzyme digestion. 2. Preparation of the vector: A vector, usually a circular DNA molecule such as a plasmid, is prepared by cleaving it with the same restriction enzymes used to isolate the gene. This creates compatible sticky ends that allow the gene to be inserted into the vector. 3. Insertion of the gene into the vector: The isolated gene is ligated into the vector using DNA ligase, forming a recombinant DNA molecule. 4. Transformation: The recombinant DNA molecule is introduced into a host organism, such as bacteria or yeast, through a process called transformation. The host organism takes up the recombinant DNA and replicates it along with its own DNA. 5. Selection and screening: Selective markers, such as antibiotic resistance genes, are often incorporated into the vector to identify host cells that have successfully taken up the recombinant DNA. Screening techniques, such as polymerase chain reaction (PCR) or DNA sequencing, are used to verify the presence of the desired gene in the host cells. Applications of Gene Cloning: Gene cloning has a wide range of applications in various fields, including: 1. Biomedical research: Gene cloning is used to study the function of specific genes and their role in diseases, leading to insights into disease mechanisms and potential therapeutic targets. 2. Genetic engineering: Gene cloning allows scientists to modify organisms for various purposes, such as enhancing crop yield, developing disease-resistant plants, or producing pharmaceuticals and vaccines. 3. Forensic science: Gene cloning techniques are employed in DNA fingerprinting and forensic analysis to identify individuals based on their unique genetic profiles. 4. Environmental monitoring: Gene cloning is used to detect and monitor environmental pollutants, pathogens, and other microorganisms in air, water, and soil samples. WhatsApp group: https://chat.whatsapp.com/HTXQC1Ax2qfKYa3MrZiMeD Twitter: https://twitter.com/basic_series Facebook Group: https://www.facebook.com/groups/742235929758671 LinkedIn: https://www.linkedin.com/in/basic-science-series-a54439208/ Instagram: https://www.instagram.com/basic_science_series/ Support my work at https://www.patreon.com/user?u=37177596 Disclaimer: The information provided is for educational purposes only. The content of this channel should not be considered as medical advice of any kind. Use this information at your own risk. We hold no responsibility for any issue, concerns, or damage arising from the content of the video. Under no circumstances this channel be responsible or liable in any way for any content, including but not limited to, any errors or omissions in the content, any loss, any damage of any kind incurred as a result of any content communicated in this video, whether by this channel or a third party. In no event shall this channel be liable for any special indirect or consequential damages of any damages whatsoever resulting from the content of the channel. ... https://www.youtube.com/watch?v=GkLVk1TJdZQ

What is Biotechnology | Scope of Biotechnology | Basic Science Series Keywords: Biotechnology, manipulation, living organisms, multidisciplinary, biology, chemistry, physics, engineering, computer science, healthcare, agriculture, industry, environmental sustainability, genetic engineering, molecular biology, bioinformatics, fermentation processes, nanotechnology, synthetic biology, sustainable development. • Biotechnology: o Manipulation of living organisms or systems for societal benefits o Multidisciplinary: Biology, chemistry, physics, engineering, computer science o Applications: Healthcare, agriculture, industry, environmental sustainability • Utilization of biological systems: o Cells, molecules for technology development o Enhances human health, food production, environmental conservation • Key applications: o Genetic engineering o Molecular biology o Tissue culture o Bioinformatics o Fermentation processes • Genetic engineering: o Modifying genetic material for specific goals o Techniques: Recombinant DNA, CRISPR-Cas9, synthetic biology • Impact in medicine: o Advancements in drug discovery, diagnostics, personalized medicine o Biopharmaceuticals: Insulin, monoclonal antibodies, vaccines • Impact in agriculture: o Transformation of crop production, livestock breeding o Genetically modified crops: Pest resistance, herbicide tolerance, improved nutrition o Industrial applications: o Bio-based chemicals, biofuels, biodegradable materials o Environmental remediation: Bioremediation • Intersection with other fields: o Nanotechnology, synthetic biology, bioinformatics • Nanobiotechnology: o Development of materials, devices, therapies at nanoscale • Potential: o Addressing global challenges o Improving human welfare o Driving sustainable development WhatsApp group: https://chat.whatsapp.com/HTXQC1Ax2qfKYa3MrZiMeD Twitter: https://twitter.com/basic_series Facebook Group: https://www.facebook.com/groups/742235929758671 LinkedIn: https://www.linkedin.com/in/basic-science-series-a54439208/ Instagram: https://www.instagram.com/basic_science_series/ Support my work at https://www.patreon.com/user?u=37177596 Disclaimer: The information provided is for educational purposes only. The content of this channel should not be considered as medical advice of any kind. Use this information at your own risk. We hold no responsibility for any issue, concerns, or damage arising from the content of the video. Under no circumstances this channel be responsible or liable in any way for any content, including but not limited to, any errors or omissions in the content, any loss, any damage of any kind incurred as a result of any content communicated in this video, whether by this channel or a third party. In no event shall this channel be liable for any special indirect or consequential damages of any damages whatsoever resulting from the content of the channel. ... https://www.youtube.com/watch?v=y0XnAuyh1g8

Learn how to use PyMOL. In this video, I will show you how to download the protein structure directly to PyMOL software. IN this video we will separate ligand from its protein structure. We will delete the protein receptor molecules and we will save the ligand as a separate PDB file. This is very useful if you want to perform docking and you are not able to find the PDB file of your molecule. PyMOL is an open-source molecular visualization system created by Warren Lyford DeLano. It was commercialized initially by DeLano Scientific LLC, which was a private software company dedicated to creating useful tools that become universally accessible to scientific and educational communities. It is currently commercialized by Schrödinger, Inc. PyMOL can produce high-quality 3D images of small molecules and biological macromolecules, such as proteins. According to the original author, by 2009, almost a quarter of all published images of 3D protein structures in the scientific literature were made using PyMOL. Official website (Download) https://pymol.org/2/ Support my work at https://www.patreon.com/user?u=37177596 Twitter: https://twitter.com/DrKumarLokender Facebook: https://www.facebook.com/lokenderkumar.sharma Linked in: https://www.linkedin.com/in/dr-lokender-kumar-58525945/ Disclaimer: The information provided is for educational purposes only. The original source of the information: https://en.wikipedia.org/wiki/PyMOL This content is available under a creative commons license: CC-BY-SA ... https://www.youtube.com/watch?v=GMz8RgKtKHI

In this video, we will know what is epidemic, endemic, and pandemic. I will also touch base on what to do in a pandemic situation. When there is a spread of disease in a given population and in a given geographical area, the experts use three basic terms to define the spread of the disease. These three terms are endemic, epidemic, and pandemic. We will discuss these terms using the example of coronavirus. What is an epidemic? An epidemic is the rapid spread of disease to a large number of people in a given population within a short period-of-time. For any infection, where the rate exceeds 15 cases per 100,000 people for two consecutive weeks is considered an epidemic. The coronavirus started as an epidemic in China because it infected a large population in a short period-of-time at a specific location i.e. china. Therefore when the infection was restricted to china it was called an epidemic. An important note is that a few cases of a very rare disease (for e.g. Rat-bite-fever) may be classified as an epidemic, while many cases of a common disease (such as the common cold) would not. Let us first discuss the term Endemic. An infection is said to be endemic when that infection is constantly maintained at a baseline level in a geographic area without any external inputs. While it might be common to say, that AIDS is "endemic" in many African countries, however, AIDS cases in Africa are increasing, so the disease is not in an endemic steady state, it is going towards becoming an epidemic. All right, now let us discuss what a pandemic is. A pandemic is an advanced form of an epidemic. An epidemic can be restricted to one geographical location; however, if it spreads to other countries or continents and affects a substantial number of people in that part of the world, it may be termed as a pandemic. In simple words, a pandemic is an epidemic occurring on a scale that crosses international boundaries, usually affecting a large number of people. This is exactly what has happened in the case of coronavirus. The virus was spread from China to other parts of the world and hence declared as a pandemic. What to do in Coronavirus pandemic situation: Do not panic and stay calm: Say calm but be alert and if possible stay inside your house if your area is under high infection alert. Keep your body and mind healthy. Keep your immune system fit: The body’s immune system is the only cure we have to the coronavirus. If your immune system is down you are at high risk. There are various ways to can keep your immune system up to date. I have made a detailed video on how to keep your immune system healthy. I will post the link in the description box. Say informed by Reliable news resource: WHO (world health Organisation) and CDC (Centers for Disease Control ... https://www.youtube.com/watch?v=JqNEnfT-mIE